Coding
CmR Backwa

Part:BBa_J31004:Design

Designed by: Erin Zwack, Sabriya Rosemond   Group: iGEM06_Davidson   (2006-07-12)


chloramphenicol acetyltransferase (backwards, CmB) [cf. BBa_J31005]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 343


Design Notes

This part was cloned into pSB1A2.

Biobricks

The Biobricks on this part are not wild type but the cut sites are still viable.

There are no spacers between the coding region and the XbaI and the SpeI sites.

GAATTC GCGGCCGC T TCTAGA - aatgcggggcgggacggtgagtagcgtcatgacaacattaagtaattcgtaagacggctgtaccttcggtagtgtttgccgtactacttggacttagcgg tcgccgtagtcgtggaacagcggaacgcatattataaacgggtaccacttttgcccccgcttcttcaacaggtataaccggtgcaaatttagttttgacc actttgagtgggtccctaaccgactctgctttttgtataagagttatttgggaaatccctttatccggtccaaaagtggcattgtgcggtgtagaacgct tatatacacatctttgacggcctttagcagcaccataagtgaggtctcgctacttttgcaaagtcaaacgagtaccttttgccacattgttcccacttgt gatagggtatagtggtcgagtggcagaaagtaacggtatgctttaaggcctactcgtaagtagtccgcccgttcttacacttatttccggcctattttga acacgaataaaaagaaatgccagaaatttttccggcattataggtcgacttgccagaccaatatccatgtaactcgttgactgactttacggagttttac aagaaatgctacggtaaccctatatagttgccaccatataggtcactaaaaaaagaggta - ACTAGT A GCGGCCG CTGCAG

EcoRI NotI Spacer XbaI Missing G spacer Coding region Missing T spacer SpeI Spacer NotI PstI

We compared our BioBricks with those from Tom Knight's paper, Idempotent Vector Design for Standard Assembly of Biobricks. As seen below

BioBricks from paper.png

Source

pSB1AC3

References

Knight, Tom. Idempotent Vector Design for Standard Assembly of Biobricks